CHEMISTRY QUESTIONS. Biological & Biomedical Sciences Coursework (Coursework Sample)

Coursework Workbook
Course Title: Bioanalytical Methods
Module Code: 66-400400-AF-20189
Level: 4
Department: Biosciences and Chemistry
Academic Year: 2018/19
Notes: This coursework provides 40% of the total assessment marks for the module and ALL questions in this workbook should be attempted. There is a total of 100 marks available in this workbook. Please use the grey spaces provided within the boxes to answer the questions. In case of any problems completing this workbook please contact the Module Leader.
Name: FORMTEXT      
Student Number: FORMTEXT      
Question 1: Explain FOUR main differences between HPLC and GC, excluding financial dissimilarities.
FORMTEXT 1. The mobile phase in the HPLC a liquid of organic solvent such as the ultrapure water while the mobile phase in the GC is a gas such as the nitrogen, helium, and argon.
2. HPLC column is short about six inches tightly packed with silica while the GC column is coiled ranging to about 100 feet depending on the need of the lab.
3. GC is suitable in analyzing volatile samples while the HPLC is ideal for analyzing less volatile compounds.
4. The temperature of the column in the GC is varied by a computer device to improve the outcome while the temperature of the HPLC is always kept constant.
(4 marks)
Question 2: What is the separation principle in Gel Filtration Chromatography?